In collaboration with the University of California, investigators at Children’s Hospital in Los Angeles have determined that the amount of a carbohydrate found in breastmilk, an oligosaccharide called 2’FL, is related to significantly higher cognitive development scores in children by the age of two years old.
 
Maternal factors, such as breast milk, have been shown to affect a baby’s development, and previous animal studies have determined that a carbohydrate found in breast milk, the oligosaccharide 2’FL, enhances cognitive development. The study performed at Children’s Hospital was the first of its kind done on humans.
 
The findings of the study, which was funded by The Gerber Foundation and the National Institutes of Health, were published on PLOS ONE.
 
In this cohort study of 50 mothers and their babies, investigators analyzed breast milk composition and frequency of feeding at 1 and 6 months of age. Cognitive development was measured at 24 months using the Bayley-III scale, a standardized test of infant and toddler development.

The study showed that the amount of 2’FL in breast milk in the first month of feeding was related to significantly higher cognitive development scores in babies by 2 years of age. The amount of 2’FL in breast milk at 6 months of feeding was not related to cognitive outcomes, indicating that early exposure may be more beneficial.
 
Many studies have reported a positive effect of breastfeeding on cognitive development. “We wanted to specifically identify what was causing this effect,” said Michael Goran, PhD, director of the Diabetes and Obesity Program at the Saban Research Institute of Children’s Hospital Los Angeles and senior author of the study.
 
“Through our high-throughput analytical platform we can quantify oligosaccharides like 2’FL and many others in hundreds of breast milk samples in a short period of time,” said study collaborator and co-author Lars Bode, PhD, professor of pediatrics and director of the Mother-Milk-Infant Center of Research Excellence at the University of California, San Diego. “This technology allows us to associate differences in milk composition with specific infant outcomes like cognitive development, validating existing data from preclinical models or generating entirely new hypotheses,” Bode added.
 
Using a statistical technique called mediation analysis, the investigators were able to independently evaluate the effects of breastfeeding in general, and the effects of the oligosaccharide 2’FL.
 
“This enhanced cognitive development in the first 2 years of life raises the question of possible long term impact on a child­–in school and beyond,” said Paige Berger, PhD, RD, a postdoctoral research associate at CHLA and the first author of the study.
 
These observations allowed the team to conclude that the increased neurodevelopment provided by breastfeeding was due primarily to mothers who were producing more 2’FL for the baby to consume.
 
“We know that there are many different compounds in breast milk and the composition is dynamic—it changes over time and is highly variable between mothers,” Goran said. “In addition to identifying the impact of oligosaccharide 2’FL, we also wanted to determine the timing of when it is most critical to a child’s development.”
 
While the investigators observed neuroenhancement explained by higher 2’FL during the first month of a baby’s life, this effect was not observed when looking at 2’FL content of breast milk at the six-month time-point. 
 
Being able to identify factors critical to early neurodevelopment offers the possibility for supplementing women’s breast milk in individuals who produce lower quantities of this important substance.
 
“For some women, breastfeeding is a challenge. For those that are not able to breastfeed or can only do so short-term, 2’FL could potentially be offered as an add-on to the nutrition their baby is receiving to better support cognitive development,” said Berger.
 
Additional study authors include Jasmine F. Plows, Roshonda B. Jones, Ji Hoon Ryoo and Bradley S. Peterson of The Saban Research Institute of Children’s Hospital Los Angeles, Marie Poulsen of the University Center for Excellence in Developmental Disabilities at CHLA, Tanya L. Alderete of the University of Colorado Boulder, and Chloe Yonemitsu and Lars Bode of the University of California, San Diego.